Journal: NPJ Microgravity

Article Title: BMP9 attenuates microgravity-related disuse osteoporosis by modulating TGFβ and BMP signaling

doi: 10.1038/s41526-025-00510-y

Figure Lengend Snippet: A Alkaline phosphatase staining. B Quantification of alkaline phosphatase enzyme activity. C Osteogenic gene expression following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). D Phosphorylation of Smads in marrow stromal cells following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, as detected by western blot. E BMP-pSmad1/5/8 signaling target gene expression in marrow stromal cells following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). ns no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: For chondrogenic differentiation, after infection with Adv-BMP9 or Adv-EGFP, TGFβ3 (10 ng/mL, Topscience, China) was added to F12/DMEM 1:1 (Gibco, USA) containing 10% fetal bovine serum (Shanghai Sangon, China) and 1% penicillin-streptomycin (Solarbio, China).

Techniques: Staining, Activity Assay, Gene Expression, Infection, Control, Virus, Phospho-proteomics, Western Blot, Targeted Gene Expression

Journal: NPJ Microgravity

Article Title: BMP9 attenuates microgravity-related disuse osteoporosis by modulating TGFβ and BMP signaling

doi: 10.1038/s41526-025-00510-y

Figure Lengend Snippet: A Phosphorylation of Smads in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, as detected by western blot. B BMP-pSmad1/5/8 signaling target gene expression in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). C Intracellular pH changes in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, normalized to control group. D Expression of osteoclastic genes in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 ( n = 3). ns no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: For chondrogenic differentiation, after infection with Adv-BMP9 or Adv-EGFP, TGFβ3 (10 ng/mL, Topscience, China) was added to F12/DMEM 1:1 (Gibco, USA) containing 10% fetal bovine serum (Shanghai Sangon, China) and 1% penicillin-streptomycin (Solarbio, China).

Techniques: Phospho-proteomics, Infection, Control, Virus, Western Blot, Targeted Gene Expression, Expressing

Journal: EBioMedicine

Article Title: Reduced circulating BMP10 and BMP9 and elevated endoglin are associated with disease severity, decompensation and pulmonary vascular syndromes in patients with cirrhosis

doi: 10.1016/j.ebiom.2020.102794

Figure Lengend Snippet: Reduced plasma BMP9 and pBMP10 levels and increased sEng levels in cirrhosis are associated with decompensation. (A,D,G) Plasma samples from controls or patients with either pre-cirrhotic liver fibrosis or with cirrhosis were assayed by ELISA for (A) BMP9, (D) pBMP10 or (G) sEng. Error bars show median and interquartile range. (B,E,H) Spearman correlation of: (B) BMP9 ( n = 69, P< 0.0001), (E) pBMP10 ( n = 64, P< 0.0001) or (H) sEng ( n = 58, P< 0.0001) levels with MELD-Na score of cirrhosis severity. (C,F,I) Patients were stratified for compensated and decompensated liver disease and plasma levels of (C) BMP9, (F) pBMP10 and (I) sEng compared between groups. Kruskal Wallis test: * P< 0.05, *** P< 0.001, **** P< 0.0001, Mann Whitney Test # P< 0.05.

Article Snippet: Primary antibodies against BMP9 (AP2064A, 1:50 Abcepta, San Diego, CA), BMP10 (LS-C293026, 1:100, LSBio, Seattle, WA) and hepatocyte antibody (OCH1E5, 1:20, Thermo Fisher, Waltham, MA) were then added to human liver slides in PBS/Tween, 3% goat serum at 250 µl per slide.

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: EBioMedicine

Article Title: Reduced circulating BMP10 and BMP9 and elevated endoglin are associated with disease severity, decompensation and pulmonary vascular syndromes in patients with cirrhosis

doi: 10.1016/j.ebiom.2020.102794

Figure Lengend Snippet: Circulating BMP9 and pBMP10 levels are reduced in PoPH and HPS, whereas sEng levels are elevated in both syndromes. Plasma samples ELISA data were for (A) BMP9, (B) pBMP10 or (C) sEng in cirrhotic patients were allocated according to the presence or absence of HPS, with controls shown for reference. Plasma samples from a separate cohort of controls and patients with confirmed PoPH were assayed by ELISA for (D) BMP9, (E) pBMP10 or (F) sEng. (G–I) The datasets for the control group samples and PoPH patients form both cohorts were combined and analysed to assess the levels of (G) BMP9, (H) pBMP10 and (I) sEng in the overall PoPH cohort. The data for the HPS patients are included in these graphs for reference. Error bars show median and interquartile range. Kruskal Wallis test: * P< 0.05, ** P< 0.01, *** P< 0.001, **** P< 0.0001, Mann Whitney Test: # P< 0.05.

Article Snippet: Primary antibodies against BMP9 (AP2064A, 1:50 Abcepta, San Diego, CA), BMP10 (LS-C293026, 1:100, LSBio, Seattle, WA) and hepatocyte antibody (OCH1E5, 1:20, Thermo Fisher, Waltham, MA) were then added to human liver slides in PBS/Tween, 3% goat serum at 250 µl per slide.

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: EBioMedicine

Article Title: Reduced circulating BMP10 and BMP9 and elevated endoglin are associated with disease severity, decompensation and pulmonary vascular syndromes in patients with cirrhosis

doi: 10.1016/j.ebiom.2020.102794

Figure Lengend Snippet: BMP activity is low in liver disease but can be reconstituted with exogenous BMP9. (A,B) HAECs were serum-depleted overnight followed by addition of 1 ng/ml BMP9, BMP10, or 1% plasma from controls ( n = 6) or liver disease patients with undetectable (320; n = 5) levels of BMP9. After 1 h, cells were lysed, RNA extracted and cDNA analysed for the expression of (A) ID1 or (B) ID2 . (C and D) Plasmas (1% final concentration) from controls ( n = 4) or liver disease patients ( n = 4) were added alone, or after spiking with ProBMP9, to serum depleted HAECs for 1 h. After 1 h, cells were lysed, RNA extracted, and cDNA analysed for the expression of (C) ID1 or (D) ID2 . (E) The same spiked plasma samples ( n = 4 control and 4 liver disease) as in (C) and (D) were assayed using the BMP9 ELISA. * P< 0.05, ** P <0.01, *** P< 0.001 Kruskal Wallis test, # P< 0.05 Mann–Whitney test compared to control plasma.

Article Snippet: Primary antibodies against BMP9 (AP2064A, 1:50 Abcepta, San Diego, CA), BMP10 (LS-C293026, 1:100, LSBio, Seattle, WA) and hepatocyte antibody (OCH1E5, 1:20, Thermo Fisher, Waltham, MA) were then added to human liver slides in PBS/Tween, 3% goat serum at 250 µl per slide.

Techniques: Activity Assay, Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: EBioMedicine

Article Title: Reduced circulating BMP10 and BMP9 and elevated endoglin are associated with disease severity, decompensation and pulmonary vascular syndromes in patients with cirrhosis

doi: 10.1016/j.ebiom.2020.102794

Figure Lengend Snippet: Liver BMP9 and BMP10 are present in hepatocytes and their expression levels are reduced in cirrhosis, whereas endoglin expression is increased . (A) Low power image (10X) of control and cirrhotic liver sections stained with haematoxylin and eosin (White scale bar length = 30 µm). (B–D) Total RNA was extracted from control ( n = 8) and cirrhotic ( n = 9) liver samples and analysed by qPCR for (B) BMP9 , (C) BMP10 and (D) ENG expression. Mann Whitney Test * P< 0.05. (E,F) 8 healthy human liver samples and 9 samples from patients with cirrhosis were stained with Hoescht nuclear stain (blue) and a hepatocyte-specific antibody (red) in addition to antibodies (green) for (E) BMP9 or (F) BMP10. Images were captured using confocal microscopy and 100 µm scale bars are shown.(For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Primary antibodies against BMP9 (AP2064A, 1:50 Abcepta, San Diego, CA), BMP10 (LS-C293026, 1:100, LSBio, Seattle, WA) and hepatocyte antibody (OCH1E5, 1:20, Thermo Fisher, Waltham, MA) were then added to human liver slides in PBS/Tween, 3% goat serum at 250 µl per slide.

Techniques: Expressing, Staining, MANN-WHITNEY, Confocal Microscopy